Application

Research CategorySignaling

Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling

Western blot 1:500 - 1:2000.

Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.

Optimal working dilutions must be determined by end user.

Anti-G Protein Giα-1 Antibody, clone R4 detects level of G Protein Giα-1 & has been published & validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Rat brain G-proteins purified by the method of Sternweis and Robishaw (Sternweis & Robishaw, 1984).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid ascites, containing sodium azide as a preservative.

Specificity

Gialpha-1. Strong reactivity with rat (100%), mouse (78%), bovine (74%) and guinea pig (48%). Slight reactivity with human (31%). Specificity confirmed by Western blot versus bovine Galphat, rat Goalpha, rat Gialpha-1, Gialpha-2, rat Gialpha-3, bovine Gsalpha, and murine GoalphaA and GoalphaB.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.